Features & Benefits
High Resolution
dPC™ chips are precision manufactured to have a difference of 0.05
pH units between adjacent gel plugs. This gives proteomic scientists the ability to fractionate and concentrate their samples by isoelectric focusing, enabling separation of proteins or peptides often found in the same fraction by other technologies.
1. Resolution up to 0.05 pH units
2. Know the pI of your protein or peptide
3. Increase proteome coverage
4. Separate highly abundant from low abundant species
5. Facilitates study of pI isoforms including post-translational modifications
Legend: The figure above shows the separation of 2 proteins Alpha 2-macroglobulin and PKDP1 by pI from plasma that was processed using the dPC™ platform.
Reproducibility
Automated manufacturing of dPC™ chips and the discrete nature of the gel features, assures that the same proteins or peptides will always be found in the same gel plugs from chip to chip and experiment to experiment.
1. Greater confidence in your data
2. Fewer replicates required
3. Eliminates variability associated with gel band cutting
System Reproducibility
Legend: High reproducibility observed using the dPC™ platform. The technical replicates of the mass spectrometer are 79%. Any variability introduced by the dPC™ chip was determined by running multiple dPC™ fractions. The ratio provides the overall reproducibility of the dPC™ platform, 92%.
Easy to Use
The dPC™ protocol is simple and straightforward. There are no complicated or tedious steps. Researchers of all disciplines will have success quickly with the system.
1. Simple straightforward protocol
2. Fewer steps, less chances for error
3. All necessary reagents are provided in the dPC™ analysis kit
Sample Enrichment
Proteins and peptides fractionated by the dPC™ become concentrated in the gel plugs relative to their concentration in the parent sample.
1. 3-fold concentration of proteins or peptides
2. Enhanced ability to detect low abundant proteins or peptides
Compatibility
The dPC™ platform is compatible with a wide array of sample types, workflows and instrumentation. The seamless integration of the technology insures that the benefits of high resolution fractionation, and reproducibility provide for an accelerated pace of discovery.
1. Compatible with both complex and simple sample types
2. Get richer, more impactful data from your current efforts
3. Confirm results with isoelectric Western Blotting iWB™
Sample Types
- Human Cell Lysates
- Bacterial Cell Lysates
- Tissue Lysates
- Yeast Cell Lysates
- Plasma
- Urine
- Cerebrospinal Fluid
- Complex Mixtures
- Proteins
- Membrane Proteins
- Peptides
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